Özet:

Objective: Common variable immunodeficiency (CVID) is a heterogeneous disease in the group of predominantly antibody deficiencies, which is defined by hypogammaglobulinemia and normal or low level of B cells, and characterized by increased susceptibility to recurrent bacterial infections, autoimmune disorders, and malignancies. In this study, we aimed to investigate the proliferation of the B and T lymphocytes in patients with CVID. Method: The proliferation of CD19 + , CD21 + , CD27 + B cells, and CD3 + , CD4 + , CD8 + T cells were investigated by Carboxyfluorescein Succinimidyl Ester (CFSE) method. CVID patients (n=13) and control group (n=5) were enrolled in the study. B and T cells were isolated from periferal blood samples with the negative selection procedure called Rosette Sep andthe isolated lymphocytes were stained in dark using CFSE. The cells were stimulated with anti- CD2, CD3, CD28 (CDmix) and cultured for four days in order to determine T cell proliferation. The proliferation of CD3 + , CD4 + , CD8 + T cells was analyzed by flow cytometry. Isolated B cells were cultured for four days along with anti-IgM+IL-4. The proliferation of CD19 + , CD27 + and CD21 + B cells were analyzed with flow cytometry.  Results: First proliferation of T lymphocytes and CD19 + B lymphocytes and second proliferation of CD4 + T lymphocytes after stimulation were increased in CVID patients compared to healthy controls.  Conclusion: In CVID patients T cell and B cells proliferation seem to be normal.

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