Özet:

Purpose: Carbapenem has become a major public health problem due to its resistance, high mortality and limited treatment options. Therefore, reliable and simple tests are needed to determine the carbapenem resistance mechanism in a short time. The purpose of this study is carbapenem resistant Klebsiella spp. and the presence of carbapenemaz, GSBL and AmpC in the E. coli isolates is determined by disc diffusion, combined disc and disc antagonism testing methods on the same plate. Method: Study with carbapenem resistant Klebsiella spp, isolated from routine patient culture samples in a university hospital between December 2017 and December 2018. This includes the Escherichia coli isolates. In accordance with the European Committee on Antimicrobial Susceptibility Testing recommendations, the combined disk method as a verification test for GSBL, KPC-type and metalobeta-lactamase and the themailin disk diffusion test for OXA-48 type carbapenemase were applied. The disc diffusion test and the disc antagonism test for indukable (chromosomal) AmpC beta-lactamate were used to identify the AmpC beta-lactamate through the plasmate. BAA-1705 K. pneumoniae, which produces KPC-2 type carbapenemase, was used as a control juice. Results: Carbapenem resistance was detected in a total of 75 isolations, including 56 (%74.6) Klebsiella pneumoniae, 17 (%22.6) E.coli, one (%1.3) Klebsiella ozaenae and one (%1.3) Klebsiella aerogenes. Metallo-beta-lactamate type carbapenemase related resistance is 24 (31.5%) isolated, GSBL 17 (22%). 3) in isolation, AmpC 4 (5.2%) in isolation and OXA-48 related resistance is determined in 31 (40.7%). The rapid detection of resistance mechanisms is important for appropriate antimicrobial treatment and infection control measures. The method we use in the study creates an easy and applicable algorithm.

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