Özet:

The cell culture method is a method developed in a biotechnological context and means that cells are cultivated in laboratory conditions and under special conditions, outside their natural environments. First of all, adult turtles entered our lives with the enlargement of nerve fibers, lymphic fluids. The needs of cell lines that are being worked from the past to the present are determined to be different from each other. Given these differences, the tissue, vasat and environmental conditions have been changed and the different cell lines and cultural methods have been found that are still in use today. Today, a variety of cell lines are used in pharmaceutical, medical and biological research. These cell lines are used to understand cell biology and tissue morphology and thus develop tissue engineering. In general, the two-dimensional cell culture model is used, but recently the three-dimensional cell culture model is also preferred. Although their foods serve different needs, it is known that some common components are extremely important for cell culture environments. When these common components are studied, especially carbohydrate sources and serum are ranked first. The main reason for this is that carbohydrates are the main energy sources, and the serum contains the necessary growth factors as well as different hormones and components. The environmental factors in which cells are located as well as the chemical components in cell culture applications are also important for cell growth and continuity. It is also necessary to show sensitivity to the topic of hygiene in cell culture methods. Because when the cultural environment, chemical components and environmental factors are treated together, the risk of contamination occurs if they do not work in aseptic conditions. This collection will provide a general view of the methods and practices of cell culture.

Anahtar Kelimeler: