Five new species of the genus Macrothele Ausserer, 1871 are described from China: Macrothele emei Lin & Li, sp. nov. (♂♀, Sichuan), M. hanfeii Lin & Li, sp. nov. (♂♀, Hainan), M. hungae Lin & Li, sp. nov. (♂♀, Taiwan), M. limenghuai Lin & Li, sp. nov. (♂♀, Sichuan), and M. nanning Lin & Li, sp. nov. (♂♀, Guangxi). Types of the new species are deposited in the Institute of Zoology, Chinese Academy of Sciences in Beijing, China.
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