Özet:

Background/aim: The genetic changes in some molecules that underlie malignant transformation of cells can be used as markers to diagnose, monitor or characterize various forms of cancer. In colorectal cancer, many genetic changes have been studied in the control of cell cycle, DNA synthesis, DNA repair, differentiation, apoptosis and tumor suppressor genes. This study aims to clarify the expression pattern of p21, p27 and p57 genes between normal and tumor colorectal tissues and correlate with clinicopathological features. Materials and methods: A total of 38 tissue samples (19 normal and 19 tumor tissue) were included in this study. The expression of p21, p27 and p57 was investigated using the RT-PCR and semi-quantitative densitometric approaches. Results: The expression levels of p21, p27 and p57 were detected to be increased in tumor tissues with respect to normal colorectal tissues. We demonstrated that the tumor differentiation of patients expressing high levels of p27 was significantly well differentiated compared to that of cases expressing low levels of p27. The clinical and pathological stages of patients expressing high levels of p57 were significantly worse than in the other patients. Conclusion: Our study suggests that the detection of expressional changes of p21, p27, and p57 might contribute to further understanding of the tumor biology of colorectal cancer and could serve as predictive markers for patient prognosis.

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