Abstract:

This study examined the changes in connective tissue elements of the uterine undergoing decidualization on the 4th, 5th, and 8th days of pregnancy by histochemical methods. The study divided 6-8 weeks-old Balb/c mice into four groups non-pregnant estrous phase, 4th, 5th, and 8th-day pregnancy models. 5µm thick sections were taken from paraffin blocks obtained from uterine tissues. Samples were stained with Hematoxylin&Eosin, Mallory Azan, Orsein, and Periodic Acid Schiff stains. As a result of the staining, the uterine tissue in the non-pregnant estrus phase showed standard histological structure. On the 4th day of pregnancy, the amount of intensely stained collagen and elastic fiber decreased on the 5th day of gestation; On the 8th day of pregnancy, it was determined that the density of the fibers increased again. As a result, both increased collagen and elastic fibers for its placement to an elastic-solid uterine tissue and increased carbohydrates for its nutrient needs and immune privilege were demonstrated in the uterus for the 4th-day embryo. The decrease in connective tissue elements with the acceleration of decidualization on the 5th and increased collagen and elastic fibers in the myometrium and the PAS + NK cells in the endometrium on the 8th-day was noted.

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