Özet:

In this study, the enzyme inhibition effects of interferon beta-1a (IFNβ-1a) have been investigated. For this purpose, enzyme inhibition effects of IFNβ-1a on human erythrocyte carbonic anhydrase isoenzyme I and II (hCA I and II) isoenzymes and acetylcholinesterase (AChE), butyrylcholinesterase (BChE) enzymes have been examined by in vitro analysis. In addition, hCA I and II isoenzymes were purified from human erythrocytes by Sepharose-4B-L-Tyrosine affinity column chromatography. Then, inhibition effects of IFNβ-1a on hCA I, II isoenzymes and AChE, BChE enzymes have been determined and IC50 and Ki values have been calculated. In this study, IC50 and Ki values have been found to be 1.73 μM and 0.78 ± 0.21 μM for hCA I isoenzyme, respectively. The same parameters have been calculated as 1.33 μM and 1.46 ± 0.13 μM for the hCA II isoenzyme. In studies on the enzyme, IC50 and Ki values have been found to be 13.86 μM and 14.47 ± 4.81 μM for AChE enzyme, respectively. Finally, in studies on the enzyme, the IC50 and Ki values were found to be 18.72 μM and 12.80 ± 4.38 μM for the BChE enzyme, respectively. In enzyme studies, acetazolamide was used for both CA isoenzymes and Tacrine was used for AChE and BChE enzymes as standard.

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