Abstract:

Example preparation; various devices (high pressure fluid chromatography, gas chromatography, radioimmun assay, spectrometric techniques, etc.) This is an important step that needs to be done prior to the analysis. For years, the extract of liquid and liquid and the subsequent removal of solvents in various forms has been the most commonly used method in the preparation of samples. But traditional liquid-fluid extraction applications have disadvantages such as being excessive amount of solvent, taking a lot of time, and being expensive. Also, these methods can cause unwanted conditions, such as the formation of the emulation phase during extraction, the obtaining of extractes that do not have the necessary purity, the failure to remove solvent sufficiently and the failure to obtain sensitive quantitative results. Other important extraction methods such as centrifugation, sliding, distillation, liophilization, in addition to liquid-fluid extraction, also have similar disadvantages. As an alternative to these methods, the solid phase extraction (SPE) method, which was a new technique, began to be used in the mid-1970s. This approach is based on the essence of designing a practical sample preparation arrangement by filling in small, single-use extraction columns of various holdings (adsorban). Solid phase extraction is used in many laboratories today as an effective sample preparation method. The solid phase extraction method is a much cheaper technique compared to the classic liquid-fluid extraction, which requires less solvent, where the emulation formation is not formed, much cheaper. In addition, a solid phase extraction can be achieved with a cleaner extract and a higher recovery rate. In this collection, solid phase extraction and other sample preparation methods; the principles, application processes and advantages of solid phase extraction; the main areas of use of solid phase extraction as well as the colon, containers and solvents used in solid phase extraction.

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