The environmental pollution is one of the major problems in the world. The decrease of agrochemicals like chemical pesticides and fertilizers is important to protect human health and the environment. The use of biocontrol agents against some root-knot nematodes Meloidogyne spp. is an alternative option to reduce environmental pollution. Paecilomyces lilacinus and Trichoderma viride are biocontrol fungi with a potential range of activity to control plant parasitic nematodes. Greenhouse experiments were conducted in 2013-2014 at the Maritsa Vegetable Crops Research Institute, Plovdiv to establish the biological activity of BioAct WG (Paecilomyces lilacinus strain 251) and Trichoderma viride strain T6 applied alone and in combination against root-knot nematodes (Meloidogyne spp.) in cucumber variety Defense F1. Two treatments with bioagents were carried out in a natural nematode population density: the first is at transplanting and the second - six weeks after it. All tested variants suppressed nematode reproduction and root galling and result in plant growth improvement compared to the control. The lowest rate of infestation and the highest total yield were established in the combination BioAct WG and Trichoderma viride strain T6. The micro-bioagents could be an effective mean to control root-knot nematodes, which results in vegetable production free of pesticides
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