Abstract:

Abstract Ampullaceana fontinalis (Studer, 1820) has not been considered as a separate species for a long time. Here we present new records of A. fontinalis for tributaries of the Siverskyi Donets River in Donetsk and Luhansk regions of Ukraine based on sequences of the cytochrome c oxidase gene and morphological characters. The newly generated sequences joined the clade of A. fontinalis and entered different subclades within it. The genetic diversity observed in the study area suggests separate evolutionary histories of geographically proximal populations of A. fontinalis. Our phylogenetic analysis confirmed species-level monophyletic groups of Ampullaceana ampla (Hartmann, 1821), Ampullaceana dipkunensis (Gundrizer & Starobogatov 1979), Ampullaceana balthica (Linnaeus, 1758), Ampullaceana lagotis (Schrank, 1803) and Ampullaceana fontinalis (Studer, 1820). The low statistical support of the latter may be attributed to the single-gene phylogeny and may be enhanced by analyzing more loci and samples.

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