Özet:

  Aim: The purpose of this study was to analyze histologically the effect of ozone therapy on alveolar bone loss and İnterleukin 1β (IL-1β), IL-10 in experimental periodontitis in a rat model. Material and Methods: Twenty-four rats were randomly divided into three experimental groups: a nonligated (NL) treatment group (n = 8), a ligature-only (LO) treatment group (n = 8), and a ligature plus ozone (60 second/each tooth a day) (LOZ) treatment group. In order to induce experimental periodontitis, a 4/0 silk suture was placed at the gingival margin of the right-mandibular first molars of the rats. The study duration was 14 days, and then the animals were sacrified. Changes in the alveolar bone levels of rats in each group were measured clinically, and the tissues of the rats in each group were examined histopathologically to determine inflammatory cell infiltration (ICI), osteoblast and osteoclast activities, and osteoclast morphology. Serum and gingival cytokine levels were measured by using the rat-specific IL-1β and IL-10 ELISA kits. Results: Alveolar bone loss around the mandibular molar tooth was significantly higher in the LO group compared with NL and LOZ groups (p<0.05). The ratio of the presence of ICI and osteoclast numbers were significantly higher in the LO group than in the NL and LOZ groups (p<0.05). Osteoblastic activity was significantly lower in the LO group than in the NL and LOZ groups (p<0.05). The serum and gingival homogenate IL-1β levels in the LO group were statistically higher than in the NL and LOZ groups (p<0.05). The serum IL-10 level in the LO group was statistically lower than in the NL and LOZ groups (p<0.05). The gingival homogenate IL-10 level in NL group was statistically higher than in the LO group (p<0.05). Conclusion: The present study showed that the oral administration of ozone diminishes alveolar bone resorption in a rat periodontitis model.  

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