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Abstract Campylobacter jejuni and C. coli are the most frequently identified thermophilic species in cases of human enteritis caused by the consumption of food products made with undercooked chicken or cross-contamination during handling of raw food products. The biochemical differentiation of C. jejuni and C. coli shows inconsistencies, such as hippurate hydrolysis and sensitivity to nalidixic acid and cephalothin. Consequently, the use of molecular methods is necessary to identify these species. The objective of this study was to identify C. jejuni and C. coli isolates from refrigerated chicken carcasses using multiplex PCR. For this purpose, the genes encoding a 160-bp subunit of oxidoreductase and the 894-bp ceuE virulence gene specific for C. jejuni and C. coli, respectively, were concurrently subjected to PCR. Of the 21 isolates assessed biochemically, 19 (90.48%) were identified as C. jejuni, one (4.76%) as C. coli, and one (4.76%) was not identified. Multiplex PCR confirmed the presence of 90.48% C. jejuni and 9.52% C. coli. The proposed approach was rapid, sensitive, and specific and is a good alternative for routine clinical testing and epidemiological studies. Author Biography Maysa Beatriz Mandetta Clementino, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz (INCQS/Fiocruz), Rio de Janeiro, RJ Possui graduação em Ciências Biológicas pela Universidade Gama Filho (1979), mestrado (1998) e Doutorado (2006) em Química Biológica pelo Instituto de Bioquímica Médica (IBqM) da Universidade Federal do Rio de Janeiro. Atualmente é Tecnologista Senior da Fundação Oswaldo Cruz e Pesquisador Colaborador do Instituto de Bioquímica Médica da UFRJ. Têm experiência na área de Microbiologia e Biologia Molecular com ênfase em Biodiversidade Microbiana, Taxonomia e Filogenia Molecular de organismos dos domínios Archaea e Bacteria de diversos ambientes. É curador da coleção de archaeas de referêcia e de archaeas isoladas no Brasil do INCQS/FIOCRUZ, atuando principalmente nos seguintes temas: Biodiversidade, Taxonomia e Filogenia Molecular de Procariotos (Archaea e Bacteria), Caraterização Molecular da Diversidade Microbiana em Ambientes Aquáticos. CV: http://lattes.cnpq.br/7459618133622863 Downloads PDF-PT (Português (Brasil)) Published 2015-08-26 How to Cite Medeiros, V. de M., Bricio, S. M. L., & Clementino, M. B. M. (2015). Identification of Campylobacter jejuni and Campylobacter coli from refrigerated chicken carcasses by Multiplex PCR. Health Surveillance under Debate: Society, Science & Technology, 3(3), 97–103. https://doi.org/10.3395/2317-269x.00363 More Citation Formats ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver Download Citation Endnote/Zotero/Mendeley (RIS) BibTeX Issue Vol. 3 No. 3 (2015): August Section Articles License Copyright (c) 2015 Health Surveillance under Debate: Society, Science & Technology (Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnología) – “Visa em Debate”

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