Özet:

SuleymanDemirel University TIP FACULTY JOURNAL: December 2004; 10(4) Experiential hyperthyroidism constitutes histological changes in adult rats' testicular tissue Meltem Özgüner, Altuğ Şenol, Mehmet Ural, Mehmet Işler Summary Purpose: Experiential hyperthyroidism in adult rats was designed to study histological changes in testicular tissue at the light microscopic level. Material and Method: 30 adult Wistar albino male mice with weight 230-280 grams were used. The group I (Control group) was 10 days serum physiological, the group II 10 days and the group III 20 days, once a day, 250 mcg/kg of L-tiroxine was injected in the subcutaneous. Thyroid function tests were studied with the osteominesanation method in the Advia Centaur autoanalyzer. After the routine follow-up guidelines for histological examination were applied, the Johnsen testicular biopsy score was calculated separately for each group in testicular tissue cuts obtained and histological evaluation was made. The findings: The difference between the groups in terms of the testicular biopsy score of Johnsen where spermogenesis was evaluated was statistically meaningful (p<0.05). In Group II and Group III, the number of germ cells and seminifer tubular histology changed, but the morphology of cells in the interstitial area did not change. Results: Adult hyperthyroidism has been observed to affect spermogenetic activity in the rats' testicles. This change was concluded that thyroid hormone could have a direct effect on germ cells and/or indirect effect through Leydig cells. Abstract Histological changes of adult rat testis in experimental hyperthyroidism: It was aimed to investigate histological changes of adult rat testis in experimental hyperthyroidism. Materials and Methods: Thirty male Wistar albino rats, weighing 230-280 gr were used. Rats in group I (Control group) were given daily s.c. injections of 0.025 N NaOH in saline, rats in group II received daily s.c. injections of 250 mcg/kg L-tiroxine for 10 days and rats in group III received daily s.c. injections of 250 mcg/kg L-tiroxine for 20 days. Thyroid function tests were quantified by Advia Centaur autoanalyzer. Histological analysis of testicular tissue including Johnsen's testicular biopsy score was performed for each group after practicing routine histological procedures. Results: Johnsen's testicular biopsy score of group II and group III were significantly decreased when com-pared to group I (p<0.05). Although germ cell morphology of group II and group III were effected, interstitial cell morphology was not effected in hyperthyroidism. Conclusion: We have observed that spermogenetic activity of adult rat testis was effected in experimental hyperthyroidism. This observation led us to conclude that it might be the result of the direct effect of thyroid hor-mones on germ cells and/or the result of indirect effect on germ cells via Leydig cells. Keywords: hyperthyroidism, testis, rat