Özet:

Introduction and Target: Anti-hemorrhagic agents already actively used in the state of health can prevent bleeding using different mechanisms of action.In this study, the effect of the mechanism of action of the "Mecsina Hemostopper", a new generation anti-hemorrhagic agent, was studied on fibroblast cell proliferation by XTT (2,3-Bis(2-metox4-nitro-5-sulfofenil)-2H-tetrazolyum) cytotoxicity analysis method.®Method and Requirements: For the study, human gingival fibroblast immortal cell lines were purchased commercially. Each bottle will be 5000 cells for each different dose group in 9 groups (Mecsina Hemostopper 1/1,Mecsina Hemostopper 1/2,Mecsina Hemostopper 1/10,Mecsina Hemostopper 1/50,Mecsina Hemostopper 1/100,Mecsina Hemostopper 1/200,Mecsina Hemostopper 1/500,Distile water applied negative and nothing applied control ) cells were distributed. After 24 hours of incubation, the cytotoxicity values were measured by the XTT analysis method for each group. The effects of Mecsina Hemostopper on the fibroblast cell have been significantly differentiated among the dosing groups (p<0,03). The most deaths were observed in concentrations of 100% and 50%, while the highest vitality was observed in the use of 10%. There was a significant difference between the concentration of 10% and the concentration of 2, 1% and 0% and the concentration of 0.2% (p<0,001). There was no significant difference between the groups in drug applications in concentrations of 2, 1, 0,5% and 0,2% (p>0,05). CONTRIBUTION and RESULTS: In this study, the new generation of hemostopper gingival fibroblast cell lines, which is a bleeding inhibitor, have different degree of cytotoxic values in different concentrations.

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