Present study on DUS characterization is about the most promising high root yielding genotype HWS 8-18 of Ashwagandha (Withania somnifera) was done during the late Kharif, 2018 and 2019 and established its distinctness among different genotypes. The genotype HWS 8-18 was tested for several morphological descriptors. The ashwagandha genotype HWS 8-18 has been found superior in dry root yield as compared to ASW-1 (best check) 10.78% at National level and 40.99% higher in the Haryana State. The mean seed yield of HWS 8-18 was 610.87 kg/ha compared to 551.43 kg/ha of ASW-1 (best check) recorded at National level while at Hisar the mean seed yield was found to be 767 kg/ha as against ASW-1 (544 kg/ha). The genotype HWS 8-18 has given numerically high seed yield (579.41 kg/ha) and at par with best check, AWS-1 (541.05 kg/ha). The morphological characters, lenceolate, smooth, medium dark green leaves, orange yellow berry colour and semi-erect are the marker characters of HWS 8-18. In addition to this, the characterization information will be helpful to maintain the genetic as well as proper identification. Moreover, high root yield and seed yield have greater importance and would be utilized in hybridization for further genetic improvement.
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