Abstract:

The purpose of this study is to investigate the inhibition effect of methylene blue by confirming some of the kinetic characteristics of the wheat tissue arginase. The wheels used in the study were provided from the Elkas cutting machine in Elazığ. Arginase activity was spectrophotometrically measured using the thyosemicarbazide-diacetylmonoxide urine (TDMU) method. Protein is measured by the method of Lowry and his friends. Four groups were used in the study. In the first group, MnCl2 was added in the first preincubation and the samples were kept in the room light. This group was recognized as a control group. In the second group, methylene blue and MnCl2 were added in the first preincubation, and the samples were exposed to 150 watt light. In the third group, the first preincubation MnCl2 was applied to the samples, adding methylene blue in the second preincubation 150 w light. In the fourth group, the first pre-incubation methyled blue in the second pre-incubation, and the MnCl2 added, the samples were subjected to 150 w light. The sheep's wing tissue was the largest loss in arginase activity in the fourth group, while the second group followed it. The lowest loss of activity was observed in the third group. In the enzyme activity, it was found to have a inhibition of 66% in room light, 60% in darkness and 72% in 150 w light. It has been found that methylene blue causes non-competitive inhibition on the dog's wave tissue arginase. Photoinactivation is believed to have occurred as a result of the changes in the imidazole groups of the hystidal remains found in the arjinase molecule.

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