Özet:

The article presents the results on selection of optimal concentrations of nutrient media components and nutrient solution at the stages of clonal micropropagation of potato varieties Lyubava, Kemerovochanin, and Tuleevskiy (actual reproduction, rooting in vitro, adaptation to ex vitro conditions). The influence of some components of the nutrient medium (sucrose, agar-agar, growth regulators, namely α-naphthyl acetic acid, β-indolyl acetic acid, and β-indolyl propionic acid) was studied at the stages of reproduction and rooting in order to obtain regenerants of the studied potato varieties. The best development of plants on nutrient medium with addition of 4 g L-1 of agar-agar was revealed. The addition of sucrose in the concentration of 3-5% contributed to the formation of more internodes. The influence of naphthyl acetic acid, β-indolyl oil, and β-indolyl propionic acids in different concentrations on the rhizogenesis of regenerating plants of three potato varieties at the stages of reproduction and establishment of different types and concentrations of auxins was studied. The researches have shown that a one-stage method of adaptation of Solanum tuberosum L. varieties of Lyubava, Kemerovochanin, and Tuleevskiy to the conditions of ex vitro cultivation with the use of hydroponic installation is characterized by efficiency and allows to receive plants with well-developed root system. The efficiency of using the hydroponic plant for adaptation of potato regenerants at the final stage of micropropagation is shown. Key words: Healing planting material; Clonal micropropagation; Potatoes; Nutrient medium; Adaptation to ex vitro conditions References Berry, J., Björkman, ??. (2008). Photosynthetic response and adaptation to temperature in higher plants. Annu. Rev. Plant Physiol, 31, 491–543. Borodaeva, Zh.A., Muratova, S.A., Kulko, S.V., Tokhtar, L.A. (2017). Influence of different sources of carbohydrate nutrition on the rhizogenesis of microtree berry crops under in vitro conditions. Scientific Bulletins of Belgorod State University, 25(274), 21–35. Butenko, R.G. (1971). Culture of the plant tissues and cells. ??oscow. Caligari, P. D. S. (2007). The canon of potato science. 5. Diploid. Dihaploid Breeding. Potato Res, 50, 223–225. Cherevchenko T.M., Lavrent'ev A.N., Ivannikov R.V. (2008). Biotechnology of tropical and subtropical plants in vitro. Kiev: Naukova Dumka. Demenko, V.I., Shestibratov, K.A., Lebedev, V.G. (2010). Rooting is the key stage of plant reproduction in vitro. Izvestia TSKHA, 1, 73-85. Demenko, V.I., Shestibratov, K.A., Lebedev, V.G. (2010). Rooting is a key stage of plant varieties in vitro. Izvestiya TSKHA, 1, 73–85. Deryabin, A.N., Trunova, T.I. (2014). Morphophysiological and biochemical characteristics of the potato plants expressing the gene of SUC2 invertase Saccharomyces cerevisiae when growing in vitro. Vestnik of Tomsk State University, 4, 150–168. Dobranszki, J. M. (2011). Mandi Induction of in vitro tuberization by shot day period and dark-treament of potato shoots grown on hormone free medium. Acta Biologica Hungarica, 44 (4), 411–420. Elaleem, K.G., Modawi, R.S., Khalafalla, M.M. (2009). Effect of cultivar and growth regulator on in vitro micropropagation of potato (Solanum tuberosum L). American-Eurasian Journal of Sustainable Agriculture, 3(3), 487–492. Ewing, E. E. (2010). Induction of tuberization in potato (pp. 25-41). In: The molecular and cellular biology of the potato. M. E. Vayda, W.D. Park (Eds.). C. A. B. Inter-national, Redwood Press Ltd., Melksham. Jacobsen, E., Hutten, R. (2006). Stacking of resistance genes in potato by cisgenesis instead of introgression breeding. Potato developments in a changing Europ: Proc. Int. Symp. On potato (Germany, Hannover, September 8–10, 2006). Wagen, Acad. Publ., Netherlands. Kalinin, F.L., Kushnir, G.P., Sarnatskaya, V.V. (1992). Technology of microclonal reproduction of plants. Kiev: Naukova dumka. Kupreichuk, N.A., I.M. Zaboronok, S.V. Sokol, S.V. (2013). Techniques of the potato productivity increase in the original seed nurseries (pp. 72-80). In: Potato industry: a collection of scientific articles. RUE "Scientific-Prakt. Center of the National Academy of Sciences of Belarus for Potato and Vegetable Growth". S.A. Turko (Ed.). Minsk, 2013. Lapshinov N.A., Kulikova, V.I., Anoshkina, L.S. (2013). Original seed production of potatoes in the conditions of the Kemerovo region (pp. 91-90). In: Potato growing: a collection of scientific articles. S.A. Turko (Ed.). Minsk: RUE "Scientific-Practical Center of the National Academy of Sciences of Belarus for Potato and Vegetable Growth". Miakisheva, E.P., Durnikin, D.A., Tavartkiladze, O.K. (2016a). Influence of vitamin morphogenesis regenerated plants potato in vitro to intensify production of elite planting material. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(2), 166–173. Miakisheva, E.P., O.K. Tavartkiladze, D.A. Durnikin, D.A. (2016b). Clonal micropropagation of potato varieties by Western Siberia selection– the new features. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(1), 375-389. Navalneva, I.A., Mironova, O.Y. (2016). Influence of phytohormones concentration on potato multiplication coefficient in vitro of the Central Black Sea Region. Innovations in agro-industrial complex: problems and prospects, 3(11), 78–84. Nugent, J. H. A. (2001). Photosynthetic water oxidation: towards a mechanism. Biochim. Biophys. Acta, 1503, 138–146. Otroshy, M., Nazarian, F., Struik, P.C. (2009). Effects of temperature fluctuation during in vitro phase on in vitro microtuber production in different cultivars of potato So-lanum tuberosum. Plant Cell Tissue and Organ Culture, 98(2), 213–218. Petrasek, J., Friml, J. (2009). Auxin transport routes in plant development. Development, 136, 2675–2688. Ryabtseva, T.V., Kulikova, V.I., Khodaeva, V.P. (2017). Assessment of nutrient media for potato varieties reproduction in vitro. International research journal, 12–3(66), 134–137. Sanders, D., Pelloux, J., Brownlee, C., Harper, J.F. (2002). Calcium at the crossroads of signaling. The Plant Cell. Supl, 401–417. Simakov, E.A., Anisimov, B.V., Elanskiy, S.N. (2010). Potato varieties grown in Russia: 2010. Annual reference edition. Moscow: Agrospas. Steudle, E. (2001). The cohesion-tension mechanism and the acquisition of water by plant roots. Ann. Rev. Plant Physiol. Plant Mol. Biol, 847–875. Tarchevsky, I. A. (2002). Signaling systems of plant cells. Moscow. Nauka. Vechernina, N.A. (2006). Conservation of biological diversity of rare, endangered species, unique forms and plant varieties by methods of biotechnology. Thesis of Doctoral Dissertation. Barnaul: Siberian Botanical Garden Center, Siberian Branch of the Russian Academy of Sciences.

Anahtar Kelimeler: