Determination of the half-lives of the radioisotopes has an importance for understanding about the nuclear structure. In this work, 100Mo(γ,n)99Mo photonuclear reaction was studied for the first time by using a clinical linear accelerator and the half-life of 99Mo radioisotope was determined. The spectrum of 99Mo radioisotope was obtained by using a gamma spectrometer with a high purity Germanium detector. Then, the spectrum was analyzed by using MAESTRO, gf3 and ROOTsoftware. It was found that, the calculated half-life value from the experimental data (64.44 h) agreed with the results of the previous studies and the reported half-life value in Nuclear Data Database, NuDat(65.98 h). As a conclusion, it has been shown that, the photonuclear reaction mechanism for medium weight radioisotopes can be studied by using a c-linac, which has suitable end point energy
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