Özet:

Mutant microalgae Nanochloropsis oculata. and Chlorella sp included in this study exhibited a high potential for energetic lipid storage as well as high growth rates comparing to the un mutants (standard), therefore, considered promising candidates for advancing the biofuel production. In addition to the exponentially increase in biomass carbohydrates, proteins and fatty acids remarkably increased after these strains were mutated by ethyl methane sulphonate (EMS), while total lipids decreased. Biochemical compositional analysis was carried out by using gas chromatography to find out concentrations of five major fatty acids: Palmitic acid, Lenolenic acid, Stearic acid, Oleic acid and Linoleic acid. In mutant N. oculata and Chlorella sp.  Lenolenic acid is the fatty acid with high percentage (11.7 and 4.8 %) respectively compared with un- mutant (0.241 and 1.824) %. Five fatty acids were selected to assess effect of the mutation, which revealed that initial concentrations of all the fatty acids before the mutation were multiplied about 48, 16, 14, 7 and 6 fold of Lenolenic, Palmitic, Oleic, Stearic and Linoleic acids respectively in N. oculata, while slightly changes occurred in the concentrations of Chlorella sp fatty acids.

Anahtar Kelimeler: