Özet:

This study is aimed at developing a single-use electrochemical DNA biosensor where the selected DNA hybridization of the Anaplasma Phagocytophilum series, a pathogen that causes infection in a fairly widespread group of hosts, is determined by volmanetric method. The basis of this electrochemical study, in which no labelling is done, is the monitoring of a double formation by measuring the upgrade signal of the Guanin base after DNA hybridization. The biosensor design involves the inozinmodification (non-guanin) prob, the immobilization of the pen graphite electroth (PGE) and the measurement of the elevation signal of the guan with differential pulse volatility (DPV), the determination of the duplex formation. In the early stages of this study, the prob series representing Anaplasma phagocytophilum was immobilized to the surface of the activated pen graphite electrots (PGE) by age adsorption method and then the presence of hybridization between the prob and the target was detected by the guan's upgrading signal observed at 1000 mV. In the optimization study, the prob skin is 25 μg/mL, the prob immobilization time is 6 minutes, the target skin is 40 μg/mL for hybridization and the hybridization time is 10 minutes. The specificity of the developed electrochemical biosensor has been tested, using target series, which are different from the base position (MM) and the location of all bases (NC). Empedimetric measurements under the Ferri/Ferro cyanide redox system using the electrochemical empedans method have also been confirmed that DNA hybridization has been performed. To determine the determination limit (DL), the target scale was used between 0.78 μM and 3.90 μM and the determination limit was 0.244 μM.

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