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Amaç: Stres karşısında enerji ve kan akımını gereken bölgelere yönlendiren Hipotalamus-Pitüiter-Adrenal (HPA) aksın yanıtları yaşlılarda bozulmaktadır. Mide, stres ve kortisol etkilerine oldukça duyarlıdır. Akut stres ve kronik stres farklı kortisol cevapları oluşturabilir. Çalışmanın amacı yaşlı dişi sıçanlarda akut ve kronik stresin mide inflamasyon belirteçleri üzerine etkisinin değerlendirilmesidir. Gereç ve Yöntem: 24-30 aylık dişi Sprague Dawley sıçanlar (n=18), kontrol, akut stres (60 dakikalık restraint stresi) ve kronik stres (14 gün tekrarlayan 60 dakikalık restraint stresi) gruplarında değerlendirildi. Stres sonrası serumda kortikosteron, mide dokularında TNF-α ve IL-10 düzeyleri ELISA yöntemi ile incelendi. Sonuçlar SPSS 24 programında One Way Anova ve post-hoc Tukey testi ile analiz edildi. Pearson korelasyon analiziyle kortikosteron düzeyleri ve sitokin düzeyleri arasındaki ilişki belirlendi, p<0,05 anlamlı kabul edildi. Bulgular: Akut stres grubunda kontrol grubuna göre serum kortikosteron düzeylerinin arttığı, kronik stres grubunda kontrole göre değişmediği görüldü. Mide TNF-α düzeylerinin akut ve kronik stres grubunda kontrole göre arttığı görüldü. Akut stres ve kontrol gruplarında kortikosteron ve TNF-α arasında pozitif korelasyon saptandı. Mide IL-10 düzeylerinde gruplar arası belirgin bir fark saptanmadı. Sonuç: Araştırmamızda yaşlı dişi sıçanlarda kronik stresin akut strese göre daha düşük kortikosteron yanıtı oluşturduğu ortaya koyulmuştur. Bu durum böbrek üstü bezinin glukokortikoid replasman yeteneğinin yaşlılarda yetersizliğiyle ilişkili olabilir. Tekrarlayan stresle akut inflamasyon bulgularının daha da artmaması, kronik strese karşı midenin adaptasyon geliştirme mekanizmalarını akla getirmiştir. Akut stresle ilişkili midedeki proinflamatuvar sitokin TNF-α düzeylerinin değişiminde kortikosteronun rolü olduğu ortaya konmuştur.
Goal: Hypothalamus-Pitüiter-Adrenal (HPA) axis reactions that direct the energy and blood flow to areas in the face of stress disrupt in the elderly. It is very sensitive to the effects of stomach, stress and cortisol. Acute stress and chronic stress can create different cortisol responses. The aim of the study is to evaluate the impact of acute and chronic stress on stomach inflammatory indicators in older female rats. Method and method: 24-30 months old female Sprague Dawley rats (n=18) were assessed in the control, acute stress (60 minutes restraint stress) and chronic stress (14 days repeated 60 minutes restraint stress) groups. Post-stress serum corticosteroid levels in the stomach tissues, TNF-α and IL-10 levels in the stomach tissues were studied by ELISA method. The results were analyzed with One Way Anova and post-hoc Tukey tests in the SPSS 24 program. The Pearson correlation analysis determined the relationship between the corticosteroid levels and the cytokine levels, p<0.05 was considered meaningful. Results: In the acute stress group, serum corticosteroid levels increased according to the control group, and in the chronic stress group, it was not seen to change according to the control group. The levels of stomach TNF-α increased according to control in the acute and chronic stress group. A positive correlation between corticosteroid and TNF-α was found in acute stress and control groups. There is no distinct difference between the levels of the stomach IL-10. Result: Our research has found that chronic stress in older female rats has a lower corticosteroid response than acute stress. This situation may be associated with an insufficiency in the ability to glucocorticoid replacement of the adrenal gland in the elderly. The no further increase in the signs of acute inflammation with repeated stress has brought into mind the mechanisms of development of abdominal adaptation to chronic stress. Corticosteroid has been shown to play a role in the change of the pro-inflammatory cytokine TNF-α levels in the abdomen associated with acute stress.
Objective: The physiological responses of the hypothalamus-pituitary-adrenal (HPA) axis, which directs energy and blood flow to required regions against stress, are impaired in the elderly. Stomach is very sensitive to stress and cortisol effects. Acute and chronic stress can produce different cortisol responses. The aim of this study was to evaluate the effect of acute and chronic stress on gastric inflammation markers in elderly female rats. Materials and Methods: Sprague Dawley female rats (n = 18) aged 24-30 months were evaluated in the control, acute stress (60-minute restraint stress) and chronic stress (restraint stress for 14 day) groups. Following stress, serum corticosterone, TNF-α and IL-10 levels in gastric tissues were examined by ELISA method. Results were analyzed by One Way Anova and post-hoc Tukey test in SPSS 24 program. Pearson correlation analysis was used and p<0.05 was considered significant. Results: Serum corticosterone levels increased in the acute stress group but did not change in the chronic stress group compared to controls. Gastric TNF-α levels increased both in the acute and chronic stress group compared to controls. There was a positive correlation between corticosterone and TNF-α in acute stress and control groups. IL-10 levels did not change in groups. Conclusion: In elderly female rats, lower corticosterone response occurred with chronic stress. This may be related to insufficiency of glucocorticoid replacement ability of adrenal gland. Markers of acute inflammation did not increase further in chronic stress due to possible adaptation mechanisms of the stomach. Corticosterone caused changes in TNF-α levels, a proinflammatory cytokine in the stomach, during acute stress.
Alan : Sağlık Bilimleri
Dergi Türü : Ulusal
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