Özet:

Aim: This study was aimed to investigate the androgenic and anti-androgenic effects of organochlorid (Endosulfan) and pyrethroid (Cypermethrin) pesticides and herbicides (2,4-D and Trifluralin), on serum LH and FSH levels and androgen-sensitive tissues in peri-pubertal rat model with Hershberger assay. Material and Method: 56 peri-pubertal 42 days old Sprague Dawley rats were used in the study. The first group was separated as sham castration. The remaining 49 rats were castrated. After 10 days recovery period, rats were divided into further seven groups: Castration group; Testosterone Propionate (TP) group; TP+Flutamide group; TP+Endosulfan group; TP+2,4-D group; TP+Cypermethrin group; TP+Trifluralin group. 24 hours after the last application, rats were decapitated and blood samples were obtained. Prostate, bulbourethral gland, vesicula seminalis and musculus levator ani (mLA) were dissected and weighed. Serum LH, FSH and testosteron levels were determined with ELISA. Result: Castration caused significant reduction in all androgen-sensitive tissue weights n(p<0.001); whereas TP application to the castrated animals recovered this tissue weights to sham group levels (p<0.001). While Endosulfan, Cypermethrin and Trifluralin caused significant decrease in bulbourethral gland weights (p<0.01); 2,4-D significantly increased vesicula seminalis weights compared to TP group (p<0.01). None of the applied test compounds caused significant differences in prostate and mLA weights compared to TP group (p>0.05). 2,4-D, Cypermethrin and Trifluralin significantly increased serum FSH levels; but the increase in serum LH levels was only significant in Trifluralin group. Conclusion: Data obtained from this study showed that 2,4-D has androjenic effects on vesicula seminalis whereas Endosulfan, Cypermethrin and Trifluralin have anti-androgenic effects on bulbourethral gland. It can be suggested that, these environmental pollutants could affect male reproductive functions negatively. 

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