Abstract:

The main aim of this study was to define salicylic impacts on the somatic embryo formation ratio. Mature embryos, obtained from seeds, were grown on Murashige and Skoog (MS) medium containing with 1 and 2 mM salicylic acid + 1 mg mL-1 2,4-D. Salicylic acid promoted the somatic embryo formation ratio in both concentrations. Green spots were seen on the callus tissues and developed easily in a salicylic acid medium. Some physiological changes were assayed in both somatic embryos and callus tissues. The results demonstrated that H2O2 and MDA were promoted in somatic embryo tissues. In outcome, it was observed in this study that in vitro salicylic acid might be used to increase somatic embryo number in callus samples of triticale.

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