Abstract:

nd. The widespread use of medicinal plants (MP) for production of biologically active substances is leading to their sharp reduction in their nature. Some of them are rare and endangered species, many plants reduced their long area of growth. One of the actual directions of biotechnology is to obtain the secondary metabolites from plant cells cultured in vitro. The development of biotechnology is based on the study of regularities of callusogenesis and accumulation of secondary metabolites in cell cultures. In this regard, the cultivation of tissues and cells of higher plants in vitro for their industrial use for producing bioactive compounds (BAC) and preserve the gene pool determines the relevance of this study. Оbjective. The aim of the work is to study of cultivation conditions and development of technology for production of BAC from the callus mass of MP. Methods. The biotechnological, physical and chemical methods have been used. Results. The scheme of sterilization of seeds with the highest emergence of the aseptic explants (93 %) and the optimum conditions of process (the medium Murasihe and Skoog, growth regulators, light and temperature regimes have selected for cultivation) has been chosen. Conclusions. We proposed optimized cultivation conditions and technological scheme for obtaining the BAC from callus mass of Gladiolus imbricatus, which allows to obtain the complex of BAC as an extract of plant cell culture.  

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