Abstract:

The highly degraded DNA content in processed food samples results in limited efficiency in detecting GMOs. Generally, conventional DNA isolation techniques from transgenic plant seeds or raw materials are available in the literature, but studies on DNA isolation techniques from processed food samples are more limited. Also, many processed food products contain genomic DNA from numerous complex plants or animal sources. This study proposed some beneficial modifications for high-quality DNA isolation of processed foods such as biscuits, cakes, crackers, corn chips, and flours. For this purpose, isolation protocols were investigated to obtain high molecular weight and quality DNA from food samples, the first step in GMO analysis to study processed foods. PCR detection was performed with soybean and corn-specific primers to control the gene region of the target organism of expected length from the obtained DNA samples and compare them with DNA isolation yields. Among the four isolation methods tested, the modified Wizard-CTAB method showed better results in most of the tested food products. Results showed that the modified Wizard-CTAB could be used for selection can be made in different food products for studies on corn and soybean specific genes and GMO detection.

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