Abstract:

Objective: Sepsis-induced acute kidney injury (sAKI) is the leading cause of renal dysfunction and mortality in intensive care unit. We must target its multidimensional pathogenesis for new treatment strategies. Therefore, we decided to reveal the protective efficacy of thiamin on sAKI in terms of its antioxidant, anti-inflammatory and mitochondrial regulatory effects. Materials and Methods: Four rat groups were formed as; healthy (HG), sepsis (SG), thiamine (TG), sepsis + thiamine (TSG) groups. In kidney tissue, oxidant [malondialdehyde (MDA), nitric oxide (NO)] and antioxidant (glutathione (GSH), catalase (CAT)] levels were measure. In serum, tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), procalcitonin (PCT) and urea, creatine (Cr), lactate were also surveyed. Results: There were significant decreases in MDA and NO levels (p<0.001, p<0.001, respectively) and increases in GSH and CAT levels (p<0.001, p<0.001, respectively) when TSG and SG groups were compared. Proinflammatory cytokine levels were significantly elevated in SG (p<0.001). In the TSG group, PCT, IL-1β and TNF-α levels were decreased compared with SG (p<0.001 p<0.001, p<0.001, respectively). In the SG group versus HG group results, lactate levels were found to be 4-fold higher (p<0.001) and urea, Cr levels were 3-4 fold higher (p<0.001, p<0.001, respectively). In the TSG group, there was an obvious decrease in lactate and urea, Cr levels compared with SG (p<0.001, p=0.002, p<0.001, respectively). Conclusion: We revealed the preventive efficacy of thiamin against sAKI by reducing the oxidant parameters and proinflammatory cytokine levels and increasing the antioxidant parameter levels with the attainment of near normal kidney functions, simultaneously. With its cheap, readily available, and good safety profile in adults, thiamin appears like a tempting adjunctive therapy for sAKI.

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