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Hakkâri İlinde Üretilen Kabakgillerde Hıyar Mozaik Virüsü’nün DAS-ELISA Yöntemi ile Tespiti
2023
Journal:  
Sinop Üniversitesi Fen Bilimleri Dergisi
Author:  
Abstract:

Bu çalışmada; Hakkâri ilinde yetiştirilen kabakgil çeşitlerinde zararlı ve ekonomik kayıplara neden olan viral etmenlerden Hıyar mozaik virüsü (Cucumber mosaic virus; CMV), DAS-ELISA (Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay) yöntemi ile serolojik olarak araştırılmıştır. Bu viral etmenin dünya genelinde ciddi ekonomik kayıplara sebep olduğu yapılan çalışmalar ile bildirilmiştir. Bu çalışmada kullanılan yaprak örnekleri, Hakkâri ilinde kabakgil üretiminin yapıldığı Kırıkdağ, Otluca ve Merzan alanlarından virüs hastalığı açısından şüpheli bulunan yapraklarda sararma, nekroz, mozaik ve şekil bozukluğu semptomları sergileyen kabakgillerden toplanmıştır. Viral belirti gösteren 7 adet karpuz (Citrullus lanatus L.), 5 adet kavun (Cucumis melo L.), 38 adet hıyar (Cucumis sativus L.), 15 adet kabak (Cucurbita sp.), 20 adet bal kabağı (Cucurbita pepo) ve 7 adet acur (Cucumis melo var. flexuosus) olmak üzere toplam 92 adet yaprak örneği Eylül 2022’de toplanmış ve örneklere CMV enfeksiyonunun tanılanması amacı ile DAS-ELISA testi uygulanmıştır. DAS-ELISA testi ile 92 örneğin 29’unda CMV tespit edilmiştir. CMV enfekteli örnek sayısı ile en fazla %31.52 oranında Otluca’da tespit edilmiştir. Kabakgil çeşitlerinde ise test edilen 7 örneğin 6’sında %85.71 ile acurda tespit edilmiştir.

Keywords:

Detection Of Cucumber Mosaic Virus Method In Cucurbits Produced In Hakkâri Using Das-elisa Method
2023
Author:  
Abstract:

In the presence of the viral agent Cucumber mosaic virus (CMV), known to cause detrimental effects and economic losses in various types of squash cultivated in Hakkâri, was investigated through serological analysis using the DAS-ELISA (Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay) method in this study. It has been reported by studies that this viral agent causes serious economic losses worldwide. The leaf samples used in the present study were collected from cucurbits, which exhibit yellowing, necrosis, mosaic, and deformity symptoms on leaves suspected of virus disease from Kırıkdağ, Otluca, and Merzan areas where cucurbit production is carried out in Hakkâri. A total of 92 leaf samples showing viral symptoms, comprising 7 watermelons (Citrullus lanatus L.), 5 melons (Cucumis melo L.), 38 cucumbers (Cucumis sativus L.), 15 zucchinis (Cucurbita pepo), 20 pumpkins (Cucurbita moschata) and 7 acrids (Cucumis melo var. flexuosus) were collected in September 2022, and DAS-ELISA test was applied to the samples for the purpose of diagnosing CMV infection. CMV was detected in 29 of 92 samples by the DAS-ELISA test. CMV was detected mostly in the Otluca region with a rate of 31.52%. Among the cucurbit varieties examined, the highest infection rate (81.71%) was found in acrid.

Keywords:

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Sinop Üniversitesi Fen Bilimleri Dergisi

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Sinop Üniversitesi Fen Bilimleri Dergisi