Özet:

Endometrial gland secretions play an important role in uterine receptivity, and further implantation they provide nourishment for the embryo. Diabetes is known to alter the secretions of certain cells. This study aimed to identify the carbohydrate content of endometrial gland secretions using Peanut Aglutinin (PNA), Wheat Germ Agglutinin (WGA) Concanavalin-A (Con-A), Soy Bean Agglutinin (SBA). Thirty-two Wistar Albino rats were divided into 4 groups of 8: diabetes and pregnancy positive, diabetes positive, pregnancy positive, control group. Samples were collected on the 5th and 7th day of pregnancy, and in the vaginally stimulated non-pregnant rats. Positive PNA staining occurred in both groups with diabetes and was negative in the other two groups. Diabetes produced marked alterations in SBA staining in the early days of pregnancy, but did not persist for long. With regard to WGA, when diabetes combined with pregnancy, changes in the secretion of the endometrial glands occur. Con- A staining showed that both pregnancy and diabetes affect the amounts of the α- mannose chain, α- chain glucose, and N-acetyl glucosamine α- chain components of endometrial gland secretions. In conclusion this study shows that both diabetes and pregnancy affect the carbohydrate content of the secretions from the endometrial glands.

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