Özet:

In this study, the morphological and molecular identification of micro-fungi in the interior air of 4 different households used is aimed at verifying the micro-fungi diversity in the interior air of the refrigerator. The research material was obtained in the first and last weeks of October, November and December 2012 by sampling 24 times from 4 different stations (two times a month) with the air sampling device (Millipore), the sampling was taken by 100 L air aspiration in one sample. In the process of sampling the diet was used Dichloran Glycerol Agar (DG 18). Isolates are obtained as pure culture and passed into the potato Dextrose Agar (PDA) foods, incubated at 25°C for 7 days and then stored at 4°C as stock culture. According to the properties of the obtained insulates, the PDA, Malt Extract Agar (MEA), Czapek Yeast Extract Agar (CYA), Czapek Yeast Extract Agar with 20% Sucrose (CY20S), Glycerol Nitrate Agar (G25N), Czapeks-Dox Agar (CZ), Creatine Sucrose Agar (CREA), Yeast Extract Sucrose Agar (YES) and DG 18 are planted and the morphological diagnoses are made using the relevant monographs. In molecular diagnosis, a series of analysis of the obtained fungal amplycones was identified in the ABI Prism 377 DNA Sequencer (Applied Biosystems, USA) using the Sanger method, using the ABI Prism Big Dye Terminator Cycle Sequencing Ready Reaction Kit, and compared with the BLAST Analysis with similar sequences in the gene bank. The percentage distribution of the diagnosed fungi was 34% Penicillium, 24% Cladosporium, 17% Alternaria, 11% Aspergillus and 9% other species.

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