Özet:

Objective Recently, there has been a significant increase in the use of food additives. Generation of possible side effects depending on the long-term consumption of these substances from birth to death has gained importance in humans. In our study, we aimed to evaluate the exposure to sodium benzoate in terms of oxidative stress and genotoxicity, especially in prepubertal rats. Material and Method In our study, 42 male rats (4 weeks-old) divided into three groups as the control (n=14), experiment 1 (sodium benzoate Acceptable Daily Intake (ADI) dose, n=14) and experiment 2 (sodium benzoate No Advers Effect Level (NOAEL)dose, n=14) groups and sodium benzoate was administration by oral gavage for 6 weeks. At the end of the study, rats were sacrified and biochemical parameters (glucose, creatinine, AST, ALT, ALP, lipid profile, total protein, albumin), total oxidant status (TOS), total antioxidant status (TAS), ischemia modified albumin (IMA) were measured, and DNA damage was evaluated by Comet analysis. Oxidative stress index (OSI) was calculated from the TOS and TAS parameters. Results ALT and creatinine levels were increased with the NOAEL dosage of sodium benzoate. In addition, TOS, IMA and OSI levels were elevated, while TAS levels were decreased in the NOAEL group. Also, NOAEL dose of sodium benzoate significantly caused DNA damage. Conclusions In our study, no adverse effect was observed in the administration of sodium benzoate at the dose of ADI in prepubertal rats. On the other hand, we found that NOAEL dose of sodium benzoate impairs kidney and liver functions and causes oxidative stress and genotoxicity.

Anahtar Kelimeler: