Özet:

Female Wistar rats were paired with their male partners in cages. The females were checked for the presence of vaginal plugs as an indication of mating and thus fertilization. On the assumption that mating occurred around midnight, the female was considered to be 0.5-day pregnant at noon that day. The pregnant females were kept in larger cages in groups at the same stage of pregnancy until use. At 9.5 days of gestation, the embryos were removed from the mother. The pregnant rat was anaesthetized with diethyl ether. Then, the animals were placed in a supine position. The abdomen opened by midline incision in the anterior wall. The uterine horns containing the concepts were excised and placed in Hank's balanced salt solution. After this stage, the procedure was carried out in a laminar-air flow cabinet. Under a dissecting microscope, the decidual tissue around the conceptus was dissected and the parietal rope sac and Reichert's membrane were removed. Embryos were transferred to 50 cc glass culture bottle that includes heat-inactivated heterological rat serum. They were gased with a mixture of O2,CO2,N2 for one minute. The bottle was sealed with a sterile silicon bung and placed in a roller incubator rotating at approximately 30 rpm at 37°C. After 48 hours culture period, which is equivalent to 11.5 days, the embryos were harvested and morphologically and biochemically.embryonic growth was estimated

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