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Çiftlik hayvanlarında son yıllarda hastalıklara karşı daha dirençli sürülerin oluşturulması için genetik yöntemlerin kullanılmasına yönelik ilgi giderek artmaktadır. Bu tür çalışmalarda immun sistemde önemli görevlerinden dolayı mannoz bağlayıcı lektin-1 (MBL-1) geni de incelenen genlerden biridir. Sunulan çalışmada araştırma materyali olarak Türkiye’de yetiştirilen yerli sığır ırklarından Zavot (n= 81, Z), Yerli Kara (n= 87, YK), Doğu Anadolu Kırmızısı (n= 72, DAK), Boz ırk (n= 54, BI), Güney Anadolu Kırmızısı (n= 44, GAK) ile Avrupa orijinli sığır ırklarından İsviçre Esmeri (n= 61, BS) ve Simental (n= 65, S) ırkı sığırlar kullanılmıştır. Çalışmada MBL-1 geninin intron 1 (1252 G>A) ve ekzon 2 bölgelerinde (2534 G>A, 2569 T>C) bulunan toplam üç tek nükleotid polimorfizmi (SNP) yönünden incelenen sığır ırklarına ait örneklerin genotiplendirilmesi hedeflenmiştir. Çalışma sonunda 1252 G>A kodlu SNP yönünden DAK ve S ırklarında (p<0.05) 2534G>A kodlu SNP yönünden YK ve BS ırklarında (p<0.001, p<0.01 sırasıyla) Hardy Weinberg (HWE) dengesinin bozulduğu görülmüştür. 2569 T>C kodlu SNP yönünden ise tüm ırkların HW dengesinde oldukları belirlenmiştir. Bu çalışma Türkiye’de yetiştirilen sığır ırklarında MBL-1 geninin intron 1 (1252 G>A) ve ekzon 2 bölgelerinde (2534 G>A, 2569 T>C) bulunan SNP’lerin araştırıldığı ilk çalışmadır. Çalışma sonunda bu SNP’ler ile önemli yetiştiricilik hastalıkları arasındaki ilişkilerin araştırıldığı çalışmaların planlanması gerektiği düşünülmüştür.
In farming animals, interest in the use of genetic methods for the creation of more disease-resistant breeds has increased in recent years. In such studies, because of its important functions in the immune system, the mannosis binding lectin-1 (MBL-1) gene is one of the genes also studied. The research material presented in the study was used from the indigenous cattle races cultivated in Turkey Zavot (n= 81, Z), Indigenous Kara (n= 87, United Nations), Eastern Anadolu Red (n= 72, DAK), Boz Race (n= 54, BI), South Anadolu Red (n= 44, GAK) and European cattle races Switzerland Esmeri (n= 61, BS) and Simental (n= 65, S) races. The study aimed at the genotyping of samples of livestock species that were studied in the direction of a single nucleotid polymorphism (SNP) in the intron 1 (1252 G>A) and exon 2 regions (2534 G>A, 2569 T>C). At the end of the study, the DAK and S races with a code of 1252 G>A (p<0.05) and the UN and BS races with a code of 2534 G>A (p<0.001, p<0.01 respectively) found the Hardy Weinberg (HWE) balance disrupted. According to the SNP code 2569 T>C, all races are in the HW balance. This study is the first study in which SNPs of the MBL-1 gene found in intron 1 (1252 G>A) and exon 2 regions (2534 G>A, 2569 T>C) in cattle races grown in Turkey were studied. At the end of the study, it was thought that the studies in which the relations between these SNPs and significant reproductive diseases were studied should be planned.
The concern in using genetic methods in forming resistant herds against diseases in farm animals has recently been increasing. Mannose-binding lectin-1 (MBL-1) gene is one of the genes searched in this kind of research because of this gene's important responsibilities in the immune system. In the presented research, Zavot (n= 81, Z), Anatolian Black (n= 87, AB), Eastern Anatolian Red (n= 72, EAR), Turkish Gray (n= 54, TG), Southern Anatolian Red (n= 44, SAR) which are the cattle breeds raised in Turkey, and Black Switzerland (n= 61, BS) and Simmental (n= 65, S) which are Europe originated cattle breeds were used as the research material. In the research, it was aimed at genotyping the samples of cattle searched in terms of total three single nucleotide polymorphism in the intron 1 (1252 G>A) and exon 2 (2534 G>A, 2569 T>C) regions of MBL-1 gene. In the end of the study, it was found that Hardy Weinberg (HWE) equilibrium broke down in EAR and S breeds (p<0.05) in terms of 1252 G>A coded SNP; in NB and BS races (p<0.001 and p<0.01 respectively) in terms of 2534G>A coded SNP. It was determined that all breeds are in HWE in terms of 2569 T>C coded SNP. This research is the first research where SNPs in intron 1 (1252 G>A) and exon 2 (2534 G>A, 2569 T>C) regions of MBL-1 gene in the cattle breeds raised in Turkey have been searched. At the end of the study, it was thought that the studies where the relationships between SNPs and important raising disease are searched should be planned.
Alan : Ziraat, Orman ve Su Ürünleri
Dergi Türü : Ulusal
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