Abstract:

Goal: osteoporosis that occurs after menopause results in a decrease in bone mass and a disruption in the structure of bone tissue. After ovarectomy, the symptoms of osteoporosis are found and bone destruction is more than bone formation, causing bone loss. ADAMs transmembran proteins play a role in the restructuring of the extracellular matrix and in many physiological processes, including embryonic development, cell migration, adezyon, angiogenesis. The aim of our study is to investigate changes in the acute period in the blood and bone tissue in the rats formed by ovarectomy using biochemical, histopatological and immunohistochemical methods. WORK AND METHOD: Healthy and female 20 adult Wistar albino was divided into 2 groups, including the control of the rats (n:10) and the bilateral ovarectomy applied (n:10). Under anesthesia, the ovaries were cut 2 cm from the part of the uterus neck, with two dorsolateral insisions, the double-sided ovaries from the uterus apex were removed with a silk thread. One week after the ovariectomy, the animal was sacrificed. Serum estrogen, calcium and alkaline phosphate values were taken blood samples from each animal to measure, after the tibia bones were removed and detected by the formalin solution, the decalcification process was undergoed and after routine histological processes the paraffin blocks were prepared, the semi-cuts were taken and painted with Hematoxyene Eozin paint for histopatological examinations. For immunohystochemical analysis, the antigen was treated with hydrogen peroxide after being taken into the citrate tampon to apply the retrival process. Anti-ADAMTS-1 primary antibodies were applied to the cuts, evaluated in a light microscope. BULGULARY: While the control group was monitoring the radio-shaped havers and volcano channels, the collagen fiber was parallel and regular to each other. In the group of ovarectomy, degenerative changes in the bone matrice were observed, a disturbance in the lamelled bone structure. From the point of view of the ADAMTS-1 expression, the control group was positive in the osteoblast and osteocytes cells and in the thin collagen fiber in the bone marrow. In the ovariectomy group, an increase in ADAMTS-1 expression in the osteoclast cells located in the degenerative area, positive expression in the osteon structures, ADAMTS-1 in the endothelial cells was followed. In terms of the estrogen values in the blood, a significant decrease was observed in the ovariectomy group according to the control group. In calcium values, the values of control and ovaryctomy groups were close to each other. Alkaline phosfatase levels were seen to rise in the ovarian group (p=0,01). In light of the data we obtain from our study, we believe that the inclusion of ADAMTS-1 proteins in the treatment of osteoporosis can support new methods of treatment.

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