User Guide
Why can I only view 3 results?
You can also view all results when you are connected from the network of member institutions only. For non-member institutions, we are opening a 1-month free trial version if institution officials apply.
So many results that aren't mine?
References in many bibliographies are sometimes referred to as "Surname, I", so the citations of academics whose Surname and initials are the same may occasionally interfere. This problem is often the case with citation indexes all over the world.
How can I see only citations to my article?
After searching the name of your article, you can see the references to the article you selected as soon as you click on the details section.
Institutional References Quartile Statistics FAQ User Guide Contact us
Institutional Application Application for Journals
Türkçe English
 Views 20
 Downloands 3
Menu
Mendeley
Endnote
COMPARISON OF BACTERIAL DNA EXTRACTION METHODS FROM STOOL SAMPLES FOR QUANTITATIVE REAL-TIME PCR ANALYSIS
2020
Journal:  
Hacettepe Journal of Biology and Chemistry
Author:  
DOI:  
10.15671/hjbc.538110
Abstract:

The most crucial step in molecular microbiology studies is choosing an appropriate procedure to obtain sufficient, high-quality DNA. Two commercially available kits (QIAamp DNA Stool Mini Kit and PSP Spin Stool DNA Plus Kit) and a modified in-house phenol-chloroform extraction method was compared for their efficiency in isolation of certain bacteria from human stool samples for Real-Time qPCR. DNA yield was significantly higher in Invitrogen when compared to phenol-chloroform method. Spiking experiments and qPCR results revealed that efficiency was in phenol-chloroform extraction was lower when compared to other two commercial kits. Commercial kits have better results in terms of DNA recovery and purity, and have more accurate results for qPCR experiments.

Keywords:

Citation Owners
Information: There is no ciation to this publication.
Similar Articles
1. Extraction Strategy for DNA Recovery from Putrefied Teeth and Skull Bone
2016


  Details
 Download PDF
2. The comparison of DNA extraction techniques in human bone and tooth samples exposed to high heat
2019


  Details
 Download PDF
3. Evaluation of the Efficacy of Five DNA Extraction Methods for the Detection of Mycobacterium tuberculosis DNA in Direct and Processed Sputum by an In-House PCR Method
2009


  Details
 Download PDF
4. PCR-SSP tekniğine dayalı laboratuvar yapımı HLA-B*27 optimizasyonu ve test kiti geliştirilmesi
2018


  Details
 Download PDF
5. Fiziksel ve Kimyasal Koşulların Çeşitli Yöntemlerle Elde Edilmiş Viral DNA’ya Etkileri
2018


  Details
 Download PDF
6. Comparison of L452R mutation variant diagnosis in SARS-COV-2 PCR positive samples with two different qPCR kits
2023


  Details
 Download PDF
Hacettepe Journal of Biology and Chemistry

Field :   Fen Bilimleri ve Matematik

Journal Type :   Uluslararası

Metrics
Article : 772
Cite : 620
Details
Hacettepe Journal of Biology and Chemistry