Abstract:

This study developed and evaluated a nanomaterial-modified ELISA system to analyze a peptide biomarker (Human VEGF). Multiwalled carbon nanotubes (MWCNTs) were used as nanomaterial-based modifiers for the capture/detection antibodies and HRP enzyme. CNTs modifications of the ELISA method at these three different binding steps were tested and compared. The most efficient modification step was concluded depending on these works’ sensitivities, linearities, and repeatabilities. We proved that CNTs could be used successfully to enhance the performances compared to the traditional ELISA method by achieving higher sensitivities and, therefore, more confident results, especially with a 0.5 pg/ml of limit of detection value. In addition, the performances of different modification steps in the commercial serum sample and the stability of all these binding steps were evaluated, and compared with the traditional ELISA method.

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