Abstract:

Purpose: Kidney ischemic/reperfusion damage (I/R) is a complicated process that causes kidney dysfunction and can go to death if no measure is taken. This study aims to investigate the effects of Ampelopsin (AMP) used in different doses in kidney I/R damage on oxidative stress and pro-inflammatory cytokines. Methods: 32 Wistar Albino male rats were randomly divided into 4 groups (n=8): Sham, I/R, I/R+AMP 80mg/kg and I/R+AMP 160mg/kg groups. In the I/R model, the renal pedicles were clamped for 60 minutes and the I/R model was completed by applied 24 hour reperfusion by removing the clamps. The AMP app was implemented just before the reperfusion was launched. The effects of AMP on oxidative and antioxidant molecules and proinflammatory cytokines have been evaluated. Results: with I/R damage the levels of total antioxidant capacity (TAK) and superoxid dismutase (SOD) decreased (p<0.001), malondialdehitis (MDA), total oxidant capacity (TOK) and myeloperoxidase (MPO) increased (p<0.001). In the AMP groups, the levels of TAK and SOD increased (p<0.001), while the levels of MDA, TAK and MPO decreased (p<0.001). The increased proinflammatory cytokine levels with I/R damage also decreased in the AMP group (p<0.001). Result: These data showed that AMP reduces kidney damage caused by oxidative stress and prevents inflammation associated with kidney I/R damage.

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