Özet:

Fusarium oxysporum f. sp.The world’s cave (Cucumis melo L.)It is one of the most important fungal diseases that limit its cultivation.The most common pathway of the disease is the soil, and it can also be transmitted with the seed.Some of the root bacteria (Plant growth promoting rhizobacteria; PGPR) [Bacillus subtilis subsp.Subtilis (B379c), Pseudomonas aeruginosa (P07-1 and P074), Pseudomonas sp.(P48-2)] and the effects of some plant activators (AuxiGro, Crop-Set and ISR-2000) have been studied comparatively to a commonly used fungicide (Maxim XL) in the fight against the disease.® Additionally, PGPRs and activators have also been evaluated to activate certain enzymes (prolin, catalase and peroxidase) important in suppression of plant diseases. The seed grinding trials found that other PGPR isolates (B379c, P07-1 and P48-2), primarily the P07-4 isolates, increased the length of the radicule-hipocotyl and the seed vitality index according to control. These isolates, F. oxysporum f. sp. Melonis (Fom-TR01) prevented miselial development by 20-29%. In their trials, PGPR and plant activator applications have been found to significantly reduce Fusarium pulmonary disease compared to control. Among the PGPR isolates, P. aeruginosa (P07-1) disease severity is positively controlled (% 61.67) compared to 35%. It is determined as the most successful isolate by reducing by 67 percent (%AUDPC 45.39). From plant activators, AuxiGro reduced disease development by 49.25% compared to positive control, while Crop-Set reduced by 41.80% and ISR-2000 by 35.82%. P07-1 isolat and AuxiGro plant activator have been identified as the two best applications that promote anti-patogenic defence enzymes (Prolin, Catalase (CAT) and Peroxidase (POX). It has been determined that the mechanism of disease-pressing PGPR is associated with stimulated resistance along with the role of plant growth regulator.

Anahtar Kelimeler:

Özet:

Fusarium wilt disease (Fusarium oxysporum f. sp. melonis) is one of the major pathogens threatening the melon (Cucumis melo L.) production worldwide. The most common way that the disease is spread to plants is via infested soil, and infected seeds may also contribute to the disease dispersal. Effects of some plant growth promoting rhizobacteria (PGPR) [Bacillus subtilis subsp. subtilis (B379c), Pseudomonas aeruginosa (P07-1 ve P074), Pseudomonas sp. (P48-2)] and some plant activators (AuxiGro, Crop-Set and  ISR-2000) were investigated in comparison to a commonly used fungicide (Maxim® XL). Also, the roles of PGPR and activators in activating some enzymes (proline, catalase and peroxidase) which are critical in suppression of plant diseases, were evaluated. In seed germination experiments, particularly P07-04, and other PGPR isolates, B379c, P07-1 and P48-2 were significantly increased radicle-hypocotyl length and seed vigor index of melon seeds, compared to control. These isolates inhibited the mycelial development of Fusarium oxysporum f. sp. melonis (Fom-TR01) at the rates of 20-29%. In pot experiments, it was determined that PGPR and plant activator treatments significantly lowered Fusarium wilt disease, compared to positive control. Among PGPR isolates, P. aeruginosa (P07-1) was the most successful isolate (AUDPC 45.39%) by lowering disease severity at a rate of 35.67% (61.67% of efficiency compared to positive control). Along with disease prevention ability, this isolate  also displayed a plant growth promoting effect. Among plant activators, AuxiGro was the most successful activator compared to positive control, with an efficiency rate of 49.25 % while 41.80% efficiency was obtained with Crop-Set and 35.82 % with ISR-2000. These two biotic and abiotic treatments (P07-1 and AuxiGro) were identified as the best defense enzyme (Proline, “Catalase” (CAT) and peroxidase (POX)) inducers against the pathogen. The disease suppression mechanism of PGPR isolates was determined to be associated with promoting the plant growth, as well as induced resistance.