Abstract:

Objective: Chemokines are factors secreted from damaged or infected tissues to induce an inflammatory and immunological reaction. Both chemokines and their receptors are considered as critical actors of tumor formation. Our study aims to investigate CCR-3 expression, which is a chemokine receptor that has recently been found to be secreted by most cancer cells, in prostatic adenocarcinoma (PCa), and reveal any possible relation between the Gleason prognostic grade and CCR-3 levels. Material Method: The study included 25 patients with PCa who underwent prostatectomy, and 25 patients with benign prostate hyperplasia (BPH). CCR-3 was detected at mRNA level by the Real-Time PCR, and at protein level by immunohistochemical (IHC) staining in both PCa and BPH patients. Results from PCa and BPH groups were compared. In PCa group, any correlation between the CCR-3 expression and Gleason prognostic grade was also searched. Results: Although CCR-3 mRNA levels in PCa group were found to be significantly higher than the BPH group (p=0.001), the difference was not significant at the protein level (p=0.205). The difference between the CCR3 expression levels of patients with different Gleason prognostic grades was not significant at both mRNA and protein levels. A statistically significant positive correlation was found between the CCR-3 IHC staining and total PSA levels (p=0.001) in PCa patients. Conclusion: We concluded that the CCR-3 mRNA levels may be useful in the diagnosis of PCa.

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