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AMAÇ: Menopoz sonrası meydana gelen osteoporoz, kemik kütlesinde azalma ve kemik dokunun yapısında bozulma ile sonuçlanır. Ovariektomi sonrası, osteoporoz bulgularına rastlanmakta ve kemik yıkımı kemik oluşumundan fazla olduğundan kemik kaybına neden olmaktadır. ADAMs transmembran proteinleri ekstrasellüler matriksin yeniden yapılandırılmasında ve embriyonik gelişim, hücre göçü, adezyon, anjiyogenez olmak üzere birçok fizyolojik süreçte rol alırlar. Çalışmamızın amacı, ovariektomi oluşturulan sıçanlarda kan ve kemik dokusunda akut dönemde meydana gelen değişikleri biyokimyasal, histopatolojik ve immünohistokimyasal yöntemlerle araştırmaktır. GEREÇ VE YÖNTEM: Sağlıklı ve dişi 20 adet erişkin Wistar albino sıçan kontrol (n:10) ve bilateral ovariektomi uygulanan grup (n:10) olmak üzere 2 gruba ayrıldı. Anestezi altında yumurtalık rahim boynu kısmından 2 cm'lik kesi yapılarak, iki dorsolateral insizyon ile uterus apeksinden çift taraflı yumurtalıklar ipek iplikle çıkarıldı. Ovariektomi den 1 hafta sonra, deney hayvanları feda edildi. Serum östrojen, kalsiyum ve alkalin fosfat değerlerini ölçmek için her hayvandan kan örnekleri alındı, tibia kemikleri çıkarılarak formalin çözeltisine alınarak tespit edildikten sonra, dekalsifiye etme işlemine tabi tutuldu ve rutin histolojik işlemlerin ardından parafin blokları hazırlandı, yarı-ince kesitleri alınarak histopatolojik incelemeler için Hematoksilen Eozin boyası ile boyandı. İmmünohistokimyasal analiz için, antijen retrival işlemini uygulamak üzere sitrat tamponuna alındıktan sonra hidrojen peroksit ile muamele edildi. Kesitlere anti-ADAMTS-1 primer antikoru uygulandı, ışık mikroskobunda değerlendirildi. BULGULAR: Kontrol grubunda radiyal tarzda dağılmış havers kanalları ve volkman kanalları izlenirken, kollajen lifler birbirine paralel ve düzenliydi. Ovariektomi grubunda ise kemik matriksinde dejeneratif değişiklikler, lamelli kemik yapısında bozulma izlendi. ADAMTS-1 ekspresyonu açısından, kontrol grubu osteoblast ve osteosit hücrelerinde ve kemik matriksindeki ince kollajen liflerde ekspresyon pozitifti. Ovariektomi grubunda, dejeneratif alanda yer alan osteoklast hücrelerinde ADAMTS-1 ekspresyonunda artış, osteon yapılarında, endotel hücrelerinde ADAMTS-1 pozitif ekspresyonu izlendi. Kanda östrojen değerleri açısından, ovariektomi grubunda kontrol grubuna göre anlamlı bir düşüş gözlendi. Kalsiyum değerlerinde ise kontrol ve ovariektomi gruplarının değerleri birbirine yakındı. Alkalen fosfataz değerlerinin ovariektomi grubunda yükseldiği görüldü(p=0,01). SONUÇ: Çalışmamız sonucu elde ettiğimiz veriler ışığında, osteoporoz tedavisine ADAMTS-1 proteinlerinin dahil edilmesinin yeni tedavi yöntemlerini destekleyebileceği düşüncesindeyiz.
Goal: osteoporosis that occurs after menopause results in a decrease in bone mass and a disruption in the structure of bone tissue. After ovarectomy, the symptoms of osteoporosis are found and bone destruction is more than bone formation, causing bone loss. ADAMs transmembran proteins play a role in the restructuring of the extracellular matrix and in many physiological processes, including embryonic development, cell migration, adezyon, angiogenesis. The aim of our study is to investigate changes in the acute period in the blood and bone tissue in the rats formed by ovarectomy using biochemical, histopatological and immunohistochemical methods. WORK AND METHOD: Healthy and female 20 adult Wistar albino was divided into 2 groups, including the control of the rats (n:10) and the bilateral ovarectomy applied (n:10). Under anesthesia, the ovaries were cut 2 cm from the part of the uterus neck, with two dorsolateral insisions, the double-sided ovaries from the uterus apex were removed with a silk thread. One week after the ovariectomy, the animal was sacrificed. Serum estrogen, calcium and alkaline phosphate values were taken blood samples from each animal to measure, after the tibia bones were removed and detected by the formalin solution, the decalcification process was undergoed and after routine histological processes the paraffin blocks were prepared, the semi-cuts were taken and painted with Hematoxyene Eozin paint for histopatological examinations. For immunohystochemical analysis, the antigen was treated with hydrogen peroxide after being taken into the citrate tampon to apply the retrival process. Anti-ADAMTS-1 primary antibodies were applied to the cuts, evaluated in a light microscope. BULGULARY: While the control group was monitoring the radio-shaped havers and volcano channels, the collagen fiber was parallel and regular to each other. In the group of ovarectomy, degenerative changes in the bone matrice were observed, a disturbance in the lamelled bone structure. From the point of view of the ADAMTS-1 expression, the control group was positive in the osteoblast and osteocytes cells and in the thin collagen fiber in the bone marrow. In the ovariectomy group, an increase in ADAMTS-1 expression in the osteoclast cells located in the degenerative area, positive expression in the osteon structures, ADAMTS-1 in the endothelial cells was followed. In terms of the estrogen values in the blood, a significant decrease was observed in the ovariectomy group according to the control group. In calcium values, the values of control and ovaryctomy groups were close to each other. Alkaline phosfatase levels were seen to rise in the ovarian group (p=0,01). In light of the data we obtain from our study, we believe that the inclusion of ADAMTS-1 proteins in the treatment of osteoporosis can support new methods of treatment.
OBJECTIVE: Postmenopausal osteoporosis results in decreased bone mass and deterioration of bone tissue. symptoms of osteopo-rosis are observed after ovarectomy and due to the fact that bone resorption is more than bone formation, it causes bone loss. ADAMs transmembrane proteins are involved in many physiological proces-ses, including reconstruction of the extracellular matrix and embr-yonic development, cell migration, adhesion, and angiogenesis. The aim of our study was to investigate the biochemical, histopathologi-cal and immunohistochemical alterations in blood values and tibia bone tissue of ovariectomized rats in the acute phase.MATERIAL AND METHODS: Twenty healthy and female adult Wis-tar albino rats were divided into two groups as control (n:10) and bilateral ovariectomy (n: 10). A 2-cm incision was performed from the ovarian cervix under anesthesia, and two dorsolateral incisions were made from the uterine apex and the bilateral ovaries were re-moved with a silk thread. One week after ovariectomy, experimental animals were sacrificed. Blood samples were taken from each ani-mal to determine serum estrogen, calcium and alkaline phosphate values. Tibia bones were removed and placed into formalin solution for fixation, then they were decalcified and paraffin blocks were pre-pared after routine histological procedures. Semi-thin sections were cut and stained with Hematoxylin-Eosin for histopathological exa-mination. For immunohistochemical analysis, the antigen retrieval process was performed with hydrogen peroxide after administrati-on to citrate buffer. Anti-ADAMTS-1 primary antibody was applied to the sections and evaluated under a light microscope.RESULTS: In the control group, while the Haversian and Volkmann canals were distributed in raider style, collagen fibers were in pa-rallel and regular. In the ovariectomy group, degenerative changes in the bone matrix, deterioration of lamellar bone structure were observed. ADAMTS-1 expression was positive in the control group osteoblast and osteocyte cells and fine collagen fibers in the bone matrix. In the ovariectomy group, increased ADAMTS-1 expression in osteoclast cells in the degenerative area, ADAMTS-1 positive exp-ression in endothelial cells was observed in osteon structures. There was a significant decrease in ovarian estrogen values in the ovarie-ctomy group compared to the control group. Calcium values of the control and ovariectomy groups were close to each other. Alkaline phosphatase levels were increased in the ovariectomy group (P = 0.01).CONCLUSIONS: In the light of data we obtained from our study, we suggest that the inclusion of ADAMTS-1 protein may support the new treatment methods in the treatment of osteoporosis.
Alan : Sağlık Bilimleri
Dergi Türü : Ulusal
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