Abstract:

With the advancement of nanotechnology, various potential applications of nanoparticles (NPs) have attracted considerable attention. In recent years, plant tissue culture applications in agricultural nanotechnology have become more popular. However, there are very few studies evaluating the effect of aluminum oxide nanoparticles (Al2O3 NPs) on enzyme activity and pigment content after plant tissue culture application. For this purpose, Ocimum basilicum callus growth effects, lipid peroxidation, hydrogen peroxide (H2O2) scavenging activity and chlorophyll content were investigated. As a result of this application, callus formation percentage and callus weight of the stem segment were found to be better than the leaf as the source of explants. The highest callus formation percentage (100%) was recorded as 741 mg stem and 324 mg leaf in (MS + 75 mg/l Al2O3) nutrient medium. In the (B5 + 75 mg/l Al2O3) nutrient medium, the stem was found to be 675 mg and the leaf 350 mg. Stress caused by Al2O3NP application was evaluated by chlorophyll and carotenoid pigment measurement.The highest Chl-a was detected at 75 mg / l Al2O3 NP concentration. The lowest total carotenoid was reported at 100 mg/l. The lowest Chl-a was detected at 25 mg / l. It was observed that the test groups treated with Al2O3 nanoparticle were significantly higher than the control group. In particular, the malondialdehit (MDA) level at 50 mg/l was quite high (7,409 times compared to control).

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