Özet:

Purpose: Alchemilla L. species are often known to be used in the treatment of diuretic, astrenic, antispazmodic, wound healing, overmenstrual and conventional diseases. Due to the rich polyphenol content they have, it has also been established that it has antioxidant, antimicrobial and anti-inflammatory effects. In this study, extracts made from the roots of the A. mollis plant from species of the gender are designed to identify their effectiveness by conducting various activity tests to determine the antioxidant and cytotoxic effects of the extract. Method: In this study, a. Methanol extract from the roots of the mollis species and the subextracts with different polarity obtained by the partition of this extrain have been identified to determine the total amount of phenol and flavonoid and antioxidant capacity of the DPPH and ABTS radicals. The cytotoxic effects of the extract on the cell lines L929 and MDA-MB-231 have also been evaluated. Results: Depending on its high phenolic content, a high antioxidant capacity has been detected in the root extract of the plant. According to the results obtained in the L929 healthy mouse fibroblast cells and in the MDA-MB-231 breast cancer cell line, the extract has not shown a toxic effect on healthy cells in a certain concentration range, while it has been found to have a toxic effect on breast cancer cells in certain doses. Result: As a result, the A. mollis plant has been identified by studies by our part that it can be an important source in the discovery of hopeful therapeutic agents in cancer treatment by inhibiting the poliferation of cancer cells with its rich phenolic compound content.

Anahtar Kelimeler:

Özet:

Aim: It is known that the herba of the species belonging to the genus of Alchemilla L. is used as a diuretic, astringent, antispasmodic, curative, cure in invalid menstruation and for convulsive purposes. It has been determined that they have antioxidant, antimicrobial and anti-inflammatory effects due to their rich polyphenol content. This study, it was aimed to determine the effectiveness of the extracts prepared from the roots of the A. mollis, one of the species belonging to the genus, by performing various activity tests to determine the antioxidant and cytotoxic effects. Method: In this study, determination of total phenol and flavonoid amount of methanol extract prepared from roots of A. mollis species and sub-extracts with different polarity obtained by fractionation of this extract and, determination of radical scavenging effects of DPPH and ABTS to obtain their antioxidant capacity was evaluated. Cytotoxic effects of these extracts in L929 and MDA-MB-231 cell lines were also evaluated. Result: High phenolic content and accordingly high antioxidant capacity were determined in the root extracts of the plant. According to the results obtained from the extracts obtained from plant roots in L929 healthy mouse fibroblast cells and MDA-MB-231 breast cancer cell line; The extracts were found to be non-toxic to healthy cells within a certain concentration range, but toxic to breast cancer cells at certain doses. Conclusion: As a result, it has been determined with our studies that the A. mollis plant can be an important source in the discovery of promising therapeutic agents in cancer treatment by inhibiting the proliferation of cancerous cells with its rich phenolic compound content.