Abstract:

The objective of this study was to evaluate in the ovary and uterus distribution of mast cells at histochemical and immunohistochemical in the diabetic rats. METHODS: In the present study 64 adult female wistar albino rats were used. Subjects have been randomly separated into 8 different groups, four of which constituted the control group while the remaining four were the actual experimental group. Diabetes was induced by intraperitoneal injection of 60 mg/kg streptozotocin; the control group received physiological saline (5 mL kg–1). At the end of this period, both groups were processed for every cycle after detecting their menstrual cycles according to the vaginal smear findings. RESULTS: Higher mast cell (MC) numbers were observed by toluidine blue staining in ovary of experimental groups, and the least dioestrous phase of the oestrous cycle compared with other diabetic groups. However, the highest mast cells were observed in metoestrous phase, and the least cells pre-oestrous phase of the oestrous cycle. Tryptase and chymase immune staining control and diabetic rats, tryptase and chymase-positive mast cells were the most intense phase of met-oestrous.CONCLUSION: As a result, depending on the estrous cycle, it has changed and that the change in the distribution of mast cells in the ovary and the uterus was found to be a significant impact of diabetes.

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