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  Citation Number 1
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Sapanca Gölü (Sakarya) Havzası Toprak ve Sedimentlerinden Streptomyces Cinsi Bakterilerin İzolasyonu, Karakterizasyonu ve Teşhisi
2019
Journal:  
Bitlis Eren Üniversitesi Fen Bilimleri Dergisi
Author:  
Abstract:

Bu çalışmada, Sakarya ili sınırlarında bulunan Sapanca Gölü havzası toprak ve sedimentlerinden Streptomyces cinsi bakterileri izole edilerek karakterizasyon ve teşhisi yapılmıştır. Toplam 6 farklı lokaliteden alınan 12 toprak numunesinin fiziko-kimyasal analizleri yapılmış ve pH’larının 6,12 ile 7,58 değerleri arasında olduğu saptanmıştır. Saflaştırılan 60 Streptomyces izolatı havasal, misel ve difüziye pigment rengi esas alınarak 4 ana gruba ayrılmıştır. Streptomyces izolatlarına, antibiyotik duyarlılığı, degredasyon aktivitesi, büyüme, azot ve karbon kaynağı kullanımı testleri uygulanmıştır. Tüm izolatların fenotipik ve biyokimyasal karakterleri belirlenerek IDENTAX bilgisayar programına bu veriler aktarılmış, cins içerisindeki pozisyonları belirlenerek teşhisleri yapılmıştır. Teşhis edilenler türler arasında Streptomyces cyaneus, Streptomyces filipinensis, Streptomyces exfoliatus, Streptomyces chromogenus, Streptomyces chromofuscus, Streptomyces griseoruber, Streptomyces antibioticus, Streptomyces lydicus, Streptomyces lavendulae ve Streptomyces purpureus türleri bulunmaktadır. Teşhisleri tamamlanan Streptomyces izolatlarının test sonuçları MVSP 3.2 bilgisayar programı ile birbirleri arasındaki benzerlik dendogramları oluşturulmuştur. Renk kümelerini temsilen seçilen 4 suşun DNA izolasyon ve dizi analizleri yapılmış ve 27F ile 1492R evrensel primerleri kullanılarak 16S rDNA geni kısmi olarak PCR ile çoğaltılmıştır. Bu dört bakterinin GenBank veri tabanındaki türlerle olan akrabalık dereceleri ve genetik pozisyonları dendogramlar şeklinde ortaya konmuştur.

Keywords:

Isolation, Characterization and Diagnosis of Streptomyces Gender Bacteria From Soil and Sediments
2019
Author:  
Abstract:

In this study, characterization and diagnosis were made by isolating Streptomyces bacteria from the soil and sediments of the Sapanca Lake basin located at the border of the Sakarya lake. In total, 12 land samples taken from 6 different locations have been physico-chemical analysed and their pH values are between 6.12 and 7.58 and are found to be between 6.12 and 7.58 and are found to be between 6.12 and 7.8 and are found to be between 6.12 and 7.8 and are found to be between 6.12 and 7.8 and are found to be between 6.12 and 7.8 and are found to be between 6.12 and 7.8 and are found to be between 6.12 and 7.8 and are found to be between 6.12 and 7.8 and are found to be between 6.12 and 7.8 and are found to be between 6.12 and 7.8 and are found to be between 6.12 and 7.8 and are found to be between 6.12 and 7.8 and are found to be between 6.12 and 7.5 and 7.5 and are found to be between 6.12 and 7.5 and 7.5 and 7.5 and are found to be found. The purified 60 Streptomyces isolate is divided into 4 main groups based on the air, the example and diffuse pigment color. Streptomyces isolates, antibiotic sensitivity, degradation activity, growth, nitrogen and carbon resource use tests have been applied. Identifying the phenotypical and biochemical characteristics of all isolates, these data were transmitted to the IDENTAX computer program, determining the positions within the species, the diagnosis was made. The diagnosed species include Streptomyces cyaneus, Streptomyces filipinensis, Streptomyces exfoliatus, Streptomyces chromogenus, Streptomyces chromofuscus, Streptomyces griseoruber, Streptomyces antibioticus, Streptomyces lydicus, Streptomyces lavendulae and Streptomyces purpureus. The diagnosis is completed with the test results of the Streptomyces isolates with the MVSP 3.2 computer program and the similarity dendograms between each other are created. The DNA isolation and series analyses of the selected 4 shells represented the colour sets were performed and partly reproduced with the 16S rDNA gene with the 27F and 1492R universal primates. The relativity degrees and genetic positions of these four bacteria with the genes in the GenBank database are shown in the form of dendograms.

Keywords:

0
2019
Author:  
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Bitlis Eren Üniversitesi Fen Bilimleri Dergisi

Field :   Fen Bilimleri ve Matematik; Mühendislik

Journal Type :   Ulusal

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Bitlis Eren Üniversitesi Fen Bilimleri Dergisi