Abstract:

Objective Ischemia reperfusion (IR) injury is caused by a sudden and temporary impairment of blood flow to a particular organ or tissue. Renal IR-induced kidney damage causes high levels of permanent damage and mortality. Dietary omega-3 fatty acids have anti-inflammatory and immune-modulating effects and are used as protective agents. Materials and Methods In the study; Protective effects of different doses of omega-3 fatty acid in ischemia reperfusion injury were investigated by biochemical and histological analyzes. 40 adult female rats were divided into 5 groups as sham control, IR, IR+100, IR+400, IR+700 (n:8). The right kidney was removed by nephrectomy in the sham control group, and the left kidney was not treated. For IR injury; the right kidney was removed by nephrectomy, and the left kidney was clamped. Omega- 3 fatty acids of 100 mg/kg, 400 mg/kg and 700 mg/ kg were administered by gastric gavage to the treatment groups for 14 days before the surgery and for 15. gastric gavage was administrated in the morning of the operation. Results According these data; with application of omega-3, histopathological examination results were correlated. It is found that, histopathological results were supported by biochemical results. There was a significant decrease in malondialdehyde (MDA) and myeloperoxidase (MPO) levels in the treated groups, while catalase (CAT) and glutathione peroxidase (GSH-Px) levels were detected to be increased. Conclusion It was concluded that administration of omega-3 fatty acids beforehand in cases that may cause ischemia and reperfusion damage in the kidney can provide a protective effect on this tissue.

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