Özet:

Dryness is an abiotic stress factor that affects the growth and productivity of the plant. Beans like grain are reduced because they are negatively affected by dry stress. Glycine-Betain (GB), a good ozmo-tolerant, is easily taken into the leaf tissues when applied exogenously in ozmotic stress conditions, ensures the continuation of photosenthetic activity and membrane integrity in chloroplasts and reduces membrane damage. It also increases the intake of K+ and chlorophyll content. In this study, the effect of 5 mM GB application on the root and body length in two species of grain with different tolerance to drought (Sultan-95, turmeric-resistant Tosunbey), the amount of chlorophyll (SPAD), connective water content, lipite peroxidation (TBARS), cell membrane passivity (HZG) and antioxidant defense system enzyme activities (peroxidase (POX), askorbat peroxidase (APX), glutation reductaze (GR), catalase (CAT)) was studied. According to our findings, with the exogenous GB application, both types are protected from chlorosis related to drought and root length inhibition. The amount of TBARS and H2O2 increased by drought due to oxidative stress in both varieties has been found to be reduced by exogenous GB application. In suppression of oxidative stress, H2O2 detoxification was found to occur with an increase in POX activities in the Tosunbey variety and an increase in APX and CAT activities in the Sultan-95. As a result, the exogenous application of GB provided protection from oxidative damage caused by dry stress in both grain varieties.

Anahtar Kelimeler:

Özet:

Drought is an abiotic stress factor that negatively affects plant growth and productivity. Drought stress reduces the yield of cereals such as wheat. Glycine-Betaine (GB), which is a good osmo-tolerant, is easily absorbed into leaf tissues when applied exogenously under osmotic stress conditions. Thus, it maintains photosynthetic activity and membrane integrity in chloroplasts and reduces membrane damage. In this study, root and stem length, chlorophyll content (SPAD), relative water content, lipid peroxidation (TBARS), cell membrane permeability (HZG), hydrogen peroxide (H2O2) and antioxidant defense system enzyme activities (peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR), catalase (CAT)) were investigated in two wheat varieties (drought tolerant, Sultan-95; drought-resistant, Tosunbey) of 5 mM GB application. According to our results, both varieties were protected from drought-related chlorosis and root length inhibition with exogenous GB application. It was determined that the amount of TBARS and H2O2 increased with drought-dependent oxidative stress in both wheat varieties decreased with exogenous GB application. In reducing oxidative stress, it was determined that H2O2 detoxification occurred with the increase in POX activities in Tosunbey variety and with the increase in APX and CAT activities in Sultan-95. As a result, exogenous GB application provided a protection from oxidative damage caused by drought stress in both wheat varieties.