The present study describes the antimicrobial activity of the leaf, seed, seed coat and fruit capsule of horse-chestnut (Aesculus hippocastanum, L.) extracts from the Ordu Province of Turkey. These extracts were first prepared with ethanol solvent. The antimicrobial activity of the extracts was then assessed using the disc diffusion method against 7 bacteria and 1 yeast. The antimicrobial test results revealed that each the crude extracts of A. hippocastanum exhibited very weakly possess antibacterial activity in the case of both Gram-positive and Gram-negative bacteria. The most sensitive microorganism to A. hippocastanum samples were Proteus vulgaris and Listeria monocytogenes. The results presented in this paper suggest the possibility that A. hippocastanum possesses a new antimicrobial compound that has a weakly effect against some Gram-negative and Gram-positive bacteria, but did not against fungi.
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